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KMID : 1041219910330010013
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Journal of Sericultural and Entomological Science
1991 Volume.33 No. 1 p.13 ~ p.20
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Characterization of Flacherie Virus of the Silkworm, Bombyx mori, isolated in Korea
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金槿榮/Kim, Keun Young
姜錫宇/姜錫權/金洛相/Kang, Suk Woo/Kang, Seok Kwon/Kim, Rack Sang
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Abstract
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The flacherie virus(FV) was isolated from the diseased larvae with flaccidity symptoms of the silkworm, Bombyx mori, which were collected in Hyangnam, Kyunggi Province and in Youcheon, Kyungpook Province. The properties of the virus were investigated and compared with the Japanese FV in morphology, size, nucleic acid and structural proteins. The Hyangnam isolate had a diameter of 27 + 1.7 nm with speherical shape and contained RNA of which the electrophoretic pattern was same as that of the Japanese FV. The virus had four structural proteins and their molecular weight were estimated as 35,000, 33,000, 31,000 and 11,400 daltons, respectively. The Youcheon isolate had two different sizes in diameter of 27 + 1.7 nm and 21 + (). 8 nm. The antigenicity of the Hyangnam isolate was proved to be identical to that of Japanese FV, whereas the antisera against the Youcheon isolate (mixed with two different sizes)reacted with Japanese FV and densonucleosis virus type 1, respectively. From these characteristics of the isolated viruses, it was concluded that the Hyangnam isolate was identical to the Japanese FV and the Youcheon isolate contained the same viruses as the Japanese FV and DNV-1.
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